名称：抗U2AF35抗体(anti-U2AF35 antibody)

　　货号：FNab09138

　　适应种属：人源

　　规格：100ug(微克)

　　状态：液体

　　纯化方法：蛋白A/G柱纯化

　　纯度：经sds-page分析，纯度大于95

　　宿主：小鼠

　　亚型：IgG1

　　存储：用添加了0.02叠氮化钠和50甘油的pbs溶解，ph值7.3，可在-20℃下保存12个月（避免反复冻融）

　　免疫原：U2 small nuclear RNA auxiliary factor 1

　　免疫原别称：DKFZp313J1712, FP793, RN, RNU2AF1, U2AF1, U2AF35, U2AFBP

　　分子量：38 kDa

　　验证应用：ELISA, WB, IHC, IP

　　建议稀释比例：WB: 1:500-1:5000; IHC: 1:20-1:200; IP: 1:200-1:2000

　　克隆性：单克隆

　　uniprot id：Q01081

　　背景介绍：U2 auxiliary factor (U2AF) , comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1, synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1) is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder。

　　Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab09138 (U2AF35 Antibody) at dilution of 1:50。

　　IP Result of anti-U2AF35 (IP:FNab09138, 3ug; Detection:FNab09138 1:500) with HeLa cells lysate 3000ug。

　　HeLa cells were subjected to SDS PAGE followed by western blot with FNab09138 (U2AF35 Antibody) at dilution of 1:1000。